Pee-wee's Big Holiday is a 2016 American adventure comedy film directed by John Lee and written by Paul Reubens and Paul Rust. The film stars Reubens as Pee-wee Herman and Joe Manganiello as himself. It is the second standalone sequel to Pee-wee's Big Adventure (1985), following Big Top Pee-wee (1988), and Reubens' final feature film role before his death in 2023.